Analysis Note

ControlRIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells

Application

Research CategorySignaling

Westerm Blot Analysis:
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.

Immunoprecipitation: 4 µg of a previous lot immunoprecipitated PDGFRβ from 500 µg of 3T3/A31 RIPA lysate.

Research Sub CategoryGrowth Factors & Receptors

This Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 05-825

Physical form

Cultured supernantant in 0.05% sodium azide

Quality

routinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells

Specificity

PDGFRβ, does not cross-react with EGF receptor

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Target description

190kDa